Journal: PLoS ONE

Article Title: Type 1 Diabetes Prevention in NOD Mice by Targeting DPPIV/CD26 Is Associated with Changes in CD8 + T Effector Memory Subset

doi: 10.1371/journal.pone.0142186

Figure Lengend Snippet: List of mAb used for the analysis of lymphocyte subsets. BD (BD Biosciences, San Jose, CA); eB (eBioscience, San Diego, CA); DN: double negative; DP: double positive; SP: single positive; T CM : central memory T cell; T EM : effector memory T cell; CD26 expression was assessed in all lymphocyte subsets.

Article Snippet: For the identification of cell subsets, cell suspension was stained with a primary antibody Rat anti-mouse CD8 (clone Lyt-2.2; MyBiosource, San Diego, CA).

Techniques: Expressing, Marker

Journal: PLoS ONE

Article Title: Type 1 Diabetes Prevention in NOD Mice by Targeting DPPIV/CD26 Is Associated with Changes in CD8 + T Effector Memory Subset

doi: 10.1371/journal.pone.0142186

Figure Lengend Snippet: Splenocytes were obtained at 4 and 6 weeks after treatment. A) Percentage of T effector memory (EM) , T central memory (CM) and naïve T lymphocytes (CD8 + and CD4 + ) in control (white circles) and treated mice (black circles). B) Representative FACS plots of the naïve (CD3 + CD4 + /CD8 + CD44 low CD62L + ), T CM (CD3 + CD4 + /CD8 + CD44 high CD62L + ) and T EM (CD3 + CD4 + /CD8 + CD44 high CD62L neg ) both for CD4 + and CD8 + T lymphocytes from spleen in treated and control mice at 4 weeks of treatment. C) MFI for CD26 expression on T EM, T CM and naïve T lymphocytes (CD8 + and CD4 + ) in control (white circles) and treated mice (black circles). Lines represent the mean of 8–10 mice. Comparison between treated and untreated mice showed an increased expression of CD26 on CD8 + T EM cells at any of the study’s time-points. (*p<0.05, **p<0.01, three way Anova).

Article Snippet: For the identification of cell subsets, cell suspension was stained with a primary antibody Rat anti-mouse CD8 (clone Lyt-2.2; MyBiosource, San Diego, CA).

Techniques: Control, Expressing, Comparison

Journal: PLoS ONE

Article Title: Type 1 Diabetes Prevention in NOD Mice by Targeting DPPIV/CD26 Is Associated with Changes in CD8 + T Effector Memory Subset

doi: 10.1371/journal.pone.0142186

Figure Lengend Snippet: T cells from PLNs were obtained from control (white circles) and treated (black circles) NOD mice at 4 weeks after treatment. A) Percentage of T effector memory (EM) , T central memory (CM) and naïve T lymphocytes (CD8 + and CD4 + ). B) MFI for CD26 expression on T EM, T CM and naïve T lymphocytes (CD8 + and CD4 + ). The expression of CD26 was significantly increased on CD4 + T EM and CD8 + T EM cells in MK626-treated mice when compared to controls. Lines represent the mean of 6 mice (*p<0.05, ***p<0.001, three way Anova).

Article Snippet: For the identification of cell subsets, cell suspension was stained with a primary antibody Rat anti-mouse CD8 (clone Lyt-2.2; MyBiosource, San Diego, CA).

Techniques: Control, Expressing

Journal: PLoS ONE

Article Title: Type 1 Diabetes Prevention in NOD Mice by Targeting DPPIV/CD26 Is Associated with Changes in CD8 + T Effector Memory Subset

doi: 10.1371/journal.pone.0142186

Figure Lengend Snippet: A) Percentage of CD8 + and CD4 + T lymphocytes infiltrating pancreatic islets in untreated (white circles) and treated (black circles) mice assessed by flow cytometry. Lines represent the mean of 6–9 mice. No statistical differences were observed between groups (Mann-Whitney test). B) Expression of CD26 determined by flow cytometry (MFI) in CD8 + and CD4 + T lymphocytes from control (white circles) and treated (black circles) mice. Lines represent the mean of 4–8 mice. (*p≤0.05, Mann-Whitney test).

Article Snippet: For the identification of cell subsets, cell suspension was stained with a primary antibody Rat anti-mouse CD8 (clone Lyt-2.2; MyBiosource, San Diego, CA).

Techniques: Flow Cytometry, MANN-WHITNEY, Expressing, Control

Journal: PLoS ONE

Article Title: Type 1 Diabetes Prevention in NOD Mice by Targeting DPPIV/CD26 Is Associated with Changes in CD8 + T Effector Memory Subset

doi: 10.1371/journal.pone.0142186

Figure Lengend Snippet: CD8 + T EM cell from control (n = 9) and treated (n = 9) mice obtained at 4 weeks of treatment were stimulated with anti–CD3 in vitro . A) Proliferative rate in CD8 + T EM lymphocytes from MK626-treated mice (black circles) and in control group (white circles). T cell proliferation was expressed as counts per minute (c.p.m) using 3 H-TdR. B) Cytokine concentration (IL-2, IL-4, IL-6, IL-10, IL-12, IL-17, IFN-γ, TNF-α and TGF-β) in supernatant from proliferation assays in treated mice (black bars) compared to control mice (white bars). Results are expressed as mean ± SEM. Mann-Whitney test was used for the evaluation of statistical significance (*p<0.05;**p<0.01).

Article Snippet: For the identification of cell subsets, cell suspension was stained with a primary antibody Rat anti-mouse CD8 (clone Lyt-2.2; MyBiosource, San Diego, CA).

Techniques: Control, In Vitro, Concentration Assay, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Type 1 Diabetes Prevention in NOD Mice by Targeting DPPIV/CD26 Is Associated with Changes in CD8 + T Effector Memory Subset

doi: 10.1371/journal.pone.0142186

Figure Lengend Snippet: A) Representative dot plots showing CFSE-T lymphocytes, stimulated with insulin-loaded mDCs, in the presence or absence of CTV-CD8 + T EM lymphocytes (ratio 1:1) and MK626 (10μM). B) Percentage of proliferating CFSE low T lymphocytes versus all T lymphocytes, with insulin-loaded mDCs (circles and triangles), in the presence of CTV-CD8 + T EM lymphocytes (triangles) and MK626 (open symbols) for 3 days. Plots show the mean (line) of three independent experiments.

Article Snippet: For the identification of cell subsets, cell suspension was stained with a primary antibody Rat anti-mouse CD8 (clone Lyt-2.2; MyBiosource, San Diego, CA).

Techniques: